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INDEXED
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AuxiliarySet
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IGVFDS2036IYZB
IGVFDS2036IYZB
released
Json view
File Set Type
gRNA sequencing
Summary
gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
Aliases
charles-gersbach:jurkats-auxiliary-set-crispra-vp64-il2ra-low-bin-rep1
Publications
Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screens
Sequencing Results (Illumina)
1 item
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI8301GIWL
fastq
reads
R1
1
Illumina MiSeq
000000000-JFKPJ
1
24 MB
Charles Gersbach, Duke
validated
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM1480DJBL
InVitroSystem
Jurkat
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO0512VHUM
charles-gersbach:jurkat-tcells-donor-1
unspecified
Homo sapiens
Related Measurement Sets
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS4954WWBW
MeasurementSet
CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
charles-gersbach:jurkats-measurement-set-crispra-vp64-il2ra-low-bin-rep1
Charles Gersbach, Duke
File Sets Using This Auxiliary Set as an Input
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS5478QWPN
AnalysisSet
CRISPR activation FACS screen targeting IL2RA: alignments
charles-gersbach:jurkats-intermediate-analysis-crispra-vp64-il2ra
Charles Gersbach, Duke
Attribution
Award
HG012053
Principal Investigator(s)
Charles Gersbach
,
Gregory Crawford
,
Tim Reddy
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke