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IGVFDS1546MEZJ

released
File Set Type
cell sorting
Summary
cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
Aliases
jesse-engreitz:auxiliary_sortparams_CRUDO_SSFA2-Auxin0hrs-FF1

Tabular Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
sample sort parameters
sample sort parameters
Jesse Engreitz, Stanford
457 B
validated

Samples

6 items
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

6 items
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinF-PCR1-Rep1_S6
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinF-PCR2-Rep1_S12
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinF-PCR3-Rep1_S60
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinF-PCR4-Rep1_S66
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinE-PCR1-Rep1_S5
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinE-PCR2-Rep1_S11
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinE-PCR3-Rep1_S59
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinE-PCR4-Rep1_S65
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinD-PCR1-Rep1_S4
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinD-PCR2-Rep1_S10
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinD-PCR3-Rep1_S58
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinD-PCR4-Rep1_S64
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinB-PCR1-Rep1_S2
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinB-PCR2-Rep1_S8
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinB-PCR3-Rep1_S56
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinB-PCR4-Rep1_S62
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinA-PCR1-Rep1_S1
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinA-PCR2-Rep1_S7
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinA-PCR3-Rep1_S55
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinA-PCR4-Rep1_S61
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinC-PCR1-Rep1_S3
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinC-PCR2-Rep1_S9
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinC-PCR3-Rep1_S57
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinC-PCR4-Rep1_S63
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford