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IGVFDS1065WJDZ

released
File Set Type
cell sorting
Summary
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 3 more measurement sets
Aliases
jesse-engreitz:auxiliary_sortparams_CRUDO_CCND1_Auxin6hrs-FF2

Tabular Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
sample sort parameters
sample sort parameters
Jesse Engreitz, Stanford
483 B
validated

Samples

6 items
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting accessible genome regions in many genomic loci

Related Measurement Sets

5 items
MeasurementSet
mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinE-PCR1-Rep1_S305
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinE-PCR2-Rep1_S311
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinE-PCR3-Rep1_S323
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinE-PCR4-Rep1_S329
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinA-PCR1-Rep1_S301
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinA-PCR2-Rep1_S307
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinA-PCR3-Rep1_S319
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinA-PCR4-Rep1_S325
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinD-PCR1-Rep1_S304
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinD-PCR2-Rep1_S310
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinD-PCR3-Rep1_S322
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinD-PCR4-Rep1_S328
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinB-PCR1-Rep1_S302
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinB-PCR2-Rep1_S308
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinB-PCR3-Rep1_S320
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinB-PCR4-Rep1_S326
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinC-PCR1-Rep1_S303
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinC-PCR2-Rep1_S309
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinC-PCR3-Rep1_S321
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinC-PCR4-Rep1_S327
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford