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IGVFDS0997MTQA

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This object is in preview status. Metadata is subject to change until this object is released.
File Set Type
gRNA sequencing
Summary
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Aliases
gary-hon:TF_Perturb_Seq_Pilot_Lenti_CM_sgRNA_LW279

Sequencing Results (Illumina)

8 items
fastq
reads
R1
1
AAC7VLGHV
2
575 MB
Gary Hon, UT
validated
fastq
reads
R2
1
AAC7VLGHV
1
670 MB
Gary Hon, UT
validated
fastq
reads
I1
1
AAC7VLGHV
1
195 MB
Gary Hon, UT
validated
fastq
reads
I2
1
AAC7VLGHV
2
195 MB
Gary Hon, UT
validated
fastq
reads
I2
1
AAC7VLGHV
1
217 MB
Gary Hon, UT
validated
fastq
reads
I1
1
AAC7VLGHV
2
179 MB
Gary Hon, UT
validated
fastq
reads
R2
1
AAC7VLGHV
2
613 MB
Gary Hon, UT
validated
fastq
reads
R1
1
AAC7VLGHV
1
621 MB
Gary Hon, UT
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW279), male, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_WTC11
male
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_CM_LW279
Gary Hon, UT

File Sets Using This Auxiliary Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
interference Perturb-seq: filtered feature barcode matrix, raw feature barcode matrix, sparse gene count matrix, unfiltered global differential expression, unfiltered local differential expression
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_CM_PrimaryAnalysis
Gary Hon, UT

Attribution

Award
HG011996
Principal Investigator(s)
Contact P.I.
Gary Hon
Lab
Gary Hon, UT