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IGVFDS0877LMZO

released
File Set Type
cell sorting
Summary
cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
Aliases
jesse-engreitz:auxiliary_sortparams_CRUDO_KITLG-Auxin6hrs-FF4

Tabular Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
sample sort parameters
sample sort parameters
Jesse Engreitz, Stanford
473 B
validated

Samples

6 items
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

6 items
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinC-PCR1_S87
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinC-PCR2_S93
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinC-PCR3_S183
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinC-PCR4_S189
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinB-PCR1_S86
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinB-PCR2_S92
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinB-PCR3_S182
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinB-PCR4_S188
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinA-PCR1_S85
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinA-PCR2_S91
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinA-PCR3_S181
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinA-PCR4_S187
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinE-PCR1_S89
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinE-PCR2_S95
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinE-PCR3_S185
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinE-PCR4_S191
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinF-PCR1_S90
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinF-PCR2_S96
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinF-PCR3_S186
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinF-PCR4_S192
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinD-PCR1_S88
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinD-PCR2_S94
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinD-PCR3_S184
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF4-BinD-PCR4_S190
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford