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IGVFDS0426GXQB

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Aliases
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF2-BinF-PCR1_S18
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF2-BinF-PCR2_S24
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF2-BinF-PCR3_S114
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF2-BinF-PCR4_S120

Sequencing Results

4 items
fastq
reads
1
Illumina HiSeq 2500
TCTGGCGA:CTCT
11 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
TACTTAGC:ATTG
31 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
TCTGGCGA:ATTG
22 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
TACTTAGC:CTCT
15 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of KITLG) modified with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF2-BinF-PCR1_S18
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF2-BinF-PCR2_S24
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF2-BinF-PCR3_S114
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF2-BinF-PCR4_S120
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford