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IGVFDS0329USVJ

released
File Set Type
cell sorting
Summary
cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
Aliases
jesse-engreitz:auxiliary_sortparams_CRUDO_KITLG-Auxin0hrs-FF3

Tabular Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
sample sort parameters
sample sort parameters
Jesse Engreitz, Stanford
490 B
validated

Samples

6 items
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

6 items
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinF-PCR1_S30
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinF-PCR2_S36
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinF-PCR3_S126
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinF-PCR4_S132
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR1_S27
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR2_S33
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR3_S123
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR4_S129
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinB-PCR1_S26
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinB-PCR2_S32
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinB-PCR3_S122
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinB-PCR4_S128
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinA-PCR1_S25
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinA-PCR2_S31
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinA-PCR3_S121
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinA-PCR4_S127
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinD-PCR1_S28
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinD-PCR2_S34
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinD-PCR3_S124
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinD-PCR4_S130
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinE-PCR1_S29
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinE-PCR2_S35
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinE-PCR3_S125
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinE-PCR4_S131
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford